GOST 32429-2013 PDF

Full title and description

ГОСТ 32429-2013 — Методы испытаний химической продукции, представляющей опасность для окружающей среды. Краткосрочное определение ингибирования ароматазы и эстрогенной и андрогенной активности: 21‑дневный тест. English title: Testing of chemicals of environmental hazard. 21‑day fish assay: a short‑term screening for oestrogenic and androgenic activity, and aromatase inhibition.

Abstract

GOST 32429-2013 specifies an in vivo, 21‑day screening assay using small teleost fish to detect estrogenic and androgenic activity and inhibition of aromatase. The standard describes test species options, exposure regimes (three test concentrations plus controls), sample collection at day 21, and biochemical and morphological endpoints such as vitellogenin (VTG) and secondary sexual characteristics. The method is intended as a short‑term screen rather than a mechanism‑specific diagnostic.

General information

• Status: Active / Введён (Действует).
• Publication date: Designation year 2013; date of introduction into force 8 January 2014 (implementation date). Some database entries record registration/metadata dates in 2013–2015.
• Publisher: Issued as a Mezhgosudarstvenny (interstate) / GOST standard administered in the EASC region; commonly published/distributed by national standards bodies and standards databases (e.g., Rosstandart/EASC catalogues).
• ICS / categories: 71.040.50 (Physico‑chemical methods of analysis / analytical chemistry).
• Edition / version: Original edition designated 2013 (ГОСТ 32429-2013).
• Number of pages: Approximately 40 pages (electronic PDF format commonly listed as 40 pp.).

Scope

The standard defines a short‑term (21‑day) in vivo fish assay to screen chemicals for oestrogenic and androgenic activity and for inhibition of aromatase. It applies to tests on small freshwater teleosts in their reproductive condition and describes exposure design, choice of concentrations, replication, feeding and husbandry, endpoints to be measured (including vitellogenin and secondary sexual characteristics), and acceptability/validity criteria for the test. The test is explicitly a screening tool and is not intended to identify detailed molecular mechanisms or to detect androgen antagonists.

Key topics and requirements

• Test duration: 21 days exposure with sample collection at day 21.
• Design: three test concentrations plus a clean control and (if needed) a solvent control.
• Replication and stocking: for medaka and zebrafish (Danio rerio) two replicates with typically five males and five females per replicate; for species such as gudgeon (as referenced in the standard) four replicates with distributions adapted to species behaviour (e.g., two males and four females per replicate).
• Endpoints: biochemical (vitellogenin/VTG) and morphological (secondary sexual characteristics) measurements; liver and blood sampling procedures specified.
• Validity criteria: overall mortality in test groups should be ≤10% at the end of exposure; dissolved oxygen should be ≥60% of saturation; selection of the maximum test concentration based on preliminary toxicity or solubility considerations (maximum tolerated concentration concept).
• Animal welfare and sampling: specified anaesthesia/euthanasia (e.g., buffered MS‑222 at recommended concentrations) and tissue handling SOPs for VTG and gonadal/tissue sampling.

Typical use and users

Used by environmental and ecotoxicology testing laboratories, contract research organisations, chemical and pesticide registrants, academic researchers studying endocrine disruption, and regulatory agencies performing hazard screening and risk assessment of chemicals that may affect aquatic organisms. The standard is applied when a short‑term in vivo screen for endocrine activity or aromatase inhibition in fish is required as part of environmental hazard assessment.

Related standards

Part of a suite of GOST standards for environmental testing and aquatic toxicity (examples in the same series include GOST 32428-2013 — 14‑day chronic toxicity test for fish, GOST 32426-2013 — duckweed growth inhibition, and other GOST 324xx‑2013 methods addressing environmental fate and aquatic effects). These related documents provide complementary chronic toxicity and biodegradation test methods.

Keywords

21‑day fish assay; endocrine disruption; oestrogenic activity; androgenic activity; aromatase inhibition; vitellogenin (VTG); medaka; zebrafish; gudgeon; ecotoxicology; GOST 32429-2013.

FAQ

Q: What is this standard?

A: ГОСТ 32429-2013 is a Russian/Mezhgosudarstvenny (GOST) test method that specifies a short‑term (21‑day) in vivo fish assay to screen chemicals for estrogenic and androgenic activity and for aromatase inhibition.

Q: What does it cover?

A: It covers test design (concentrations, replication, species options), husbandry and exposure conditions, sample collection at day 21, analytical/biochemical and morphological endpoints (e.g., VTG and secondary sexual characteristics), validity criteria (mortality, oxygen, concentration selection) and animal handling/ euthanasia procedures. The method is intended for screening rather than mechanistic identification.

Q: Who typically uses it?

A: Contract testing laboratories, environmental toxicologists, chemical registrants and manufacturers, academic researchers in ecotoxicology and endocrinology, and regulatory authorities conducting hazard screening of chemicals that may affect aquatic organisms.

Q: Is it current or superseded?

A: As of available national/standards database records the standard is listed as active (in force) with designation 2013 and implementation date in early 2014; no replacement or cancellation is indicated in the referenced registries. Users should check the relevant national standards catalogue or publisher for the absolute latest status before formal regulatory use.

Q: Is it part of a series?

A: Yes. It belongs to a group of GOST methods addressing testing of chemicals hazardous to the environment (several GOST 3242x‑2013 standards cover fish chronic tests, plant tests, biodegradation and sediment transformation). These complementary standards are commonly used together for aquatic hazard and fate assessment.

Q: What are the key keywords?

A: 21‑day fish assay, endocrine disruption screening, vitellogenin, secondary sexual characteristics, aromatase inhibition, medaka, zebrafish, GOST 32429-2013.