GOST R 52816-2007 PDF
Name in English:
GOST R 52816-2007
Name in Russian:
ГОСТ Р 52816-2007
Food products. Methods for detection and quantity determination of coliforms
Full title and description
GOST R 52816-2007 — "Food products. Methods for detection and quantity determination of coliformes" (Russian: ГОСТ Р 52816-2007 — Продукты пищевые. Методы выявления и определения количества бактерий группы кишечных палочек (колиформных бактерий)). The document specifies laboratory methods for detection and enumeration of bacteria of the coliform group in food (except milk and dairy products), including the most probable number (MPN) technique and colony-count techniques on selective media.
Abstract
This national standard defines procedures for detecting coliform bacteria in defined sample weights/volumes and three principal enumeration methods (MPN and colony-count techniques using selective agar media). It is a modified adoption of ISO horizontal methods (ISO 4831 and ISO 4832) adapted for application in Russian food microbiology laboratories and for regulatory and quality control use.
General information
- Status: Withdrawn / replaced (replaced by GOST 31747-2012; standard entry superseded).
- Publication date: Introduced 1 January 2009 (approved by Rosstandart order dated 27 December 2007).
- Publisher: Federal Agency for Technical Regulation and Metrology (ROSTANDART) / official national publication channels (registered and published under the GOST R system).
- ICS / categories: 07.100.30 (Food microbiology).
- Edition / version: GOST R 52816-2007 (with errata published in 2009 in some distributions).
- Number of pages: Approximately 18–20 pages depending on the published edition/translation.
Scope
The standard applies to food products other than milk and dairy products. It establishes a method to detect coliforms in a specified sample mass/volume and three methods to determine their quantity: the most probable number (MPN) method (for low numbers) and colony-count techniques involving inoculation on or into agar selective-diagnostic media (for higher counts). The standard includes incubation and confirmation steps (including biochemical confirmation where necessary) and references the relevant ISO horizontal methods upon which it is based.
Key topics and requirements
- Definition and scope: coliform group bacteria in food (excludes milk/dairy in this document).
- Detection procedures: enrichment and selective media to detect presence/absence in a defined sample aliquot.
- Enumeration methods: (1) Most Probable Number (MPN) technique for low counts; (2) Colony-count (plate/agar) techniques for higher counts; (3) Alternative agar-based plating/inoculation methods with confirmatory testing.
- Incubation conditions, interpretation of gas/ turbidity indicators in MPN tubes, and colony morphology criteria for presumptive identification.
- Confirmatory tests: biochemical and/or culture characteristics to verify coliform identity when required.
- Reference and harmonization: modified adoption of ISO 4831 and ISO 4832 horizontal methods with national adjustments.
Typical use and users
Used by accredited food microbiology laboratories, quality-control units in food manufacturing, regulatory inspection bodies, and research laboratories performing routine testing or compliance verification for hygienic and safety parameters. Laboratories apply the methods for product testing, process monitoring and regulatory compliance prior to the replacement standard (GOST 31747-2012) taking effect.
Related standards
Related and referenced documents include ISO 4831 (Most probable number technique) and ISO 4832 (Colony-count technique), national standards in the GOST family such as GOST 31747-2012 (which replaced GOST R 52816-2007), and general microbiological practice standards (for example GOST ISO 7218 / ISO 7218). The standard is frequently cited alongside other Russian food microbiology standards and test-method specifications.
Keywords
coliforms, coliform bacteria, food microbiology, most probable number (MPN), colony-count, selective media, enrichment, agar plating, enumeration, detection, GOST R 52816-2007.
FAQ
Q: What is this standard?
A: GOST R 52816-2007 is a Russian national standard specifying laboratory methods for detection and enumeration of coliform bacteria in food products (excluding milk and dairy).
Q: What does it cover?
A: It covers sample inoculation, enrichment, incubation, interpretation of MPN tubes (gas/turbidity), colony-count techniques on selective agar, and confirmatory biochemical/cultural tests for coliform identification; it implements modified ISO horizontal methods for national use.
Q: Who typically uses it?
A: Accredited microbiology laboratories, food manufacturers’ QC labs, regulatory inspectors, and researchers involved in food safety testing and compliance.
Q: Is it current or superseded?
A: It has been superseded — GOST R 52816-2007 was replaced by GOST 31747-2012; users should consult GOST 31747-2012 (and current national/regional technical regulations) for up-to-date procedures.
Q: Is it part of a series?
A: It is part of the suite of food microbiology standards (ICS 07.100.30) and is linked to other GOST/GOST R documents that set general microbiological practice and test methods (for example GOST ISO 7218 and related national microbiology standards).
Q: What are the key keywords?
A: Coliforms; coliform bacteria; MPN; colony-count; selective media; food microbiology; enumeration; detection; GOST R 52816-2007.