GOST 30518-97 PDF

Full title and description

GOST 30518-97 — "Продукты пищевые. Методы выявления и определения количества бактерий группы кишечных палочек (колиформных бактерий)" / "Food products. Methods for detection and quantity determination of coliformes."

Abstract

This national standard specifies procedures for detecting presence/absence and for quantitative determination of bacteria of the coliform (coli) group in food products. It defines sample preparation, three quantitative approaches (most probable number – MPN, and two plating/enumeration methods using selective diagnostic agar media), incubation conditions and interpretation of results for routine microbiological control of food.

General information

• Status: Withdrawn / not in force in the Russian Federation (утратил силу).
• Publication date: Designation year 1997 (GOST 30518-97); introduced into force 16 April 1998 (date of entry into force reported in standards registries).
• Publisher: Approved by the State Committee for Standardization and Metrology of the Russian Federation (Gosstandart, later Rosstandart).
• ICS / categories: Microbiology / Food microbiology — classification commonly listed under 07.100.30 (food microbiology).
• Edition / version: Original national standard GOST 30518-97 (the document is normally cited as the 1997 edition; commercial reprints/reissues are recorded in some catalogs).
• Number of pages: 7 pages (typical bibliographic listings).

Scope

The standard applies to food products and establishes: methods for detecting the presence of coliform bacteria in a defined sample mass, and three methods for determining their quantity — the most probable number (MPN) technique and plating/enumeration on selective diagnostic agar media. It is intended for use in routine microbiological control and testing of foods.

Key topics and requirements

• Definitions and classification of bacteria of the coliform (coli) group.
• Sampling rules and sample mass/preparation for different food matrices.
• Presence/absence detection procedures for a specified sample portion.
• Quantitative determination methods: most probable number (MPN) and two plating/enumeration methods on selective media (incubation conditions and colony interpretation).
• Quality control, interpretation of positive/negative results, and reporting format for routine laboratory use.

Typical use and users

Used by food microbiology laboratories, quality control departments in food production, regulatory and public health laboratories, research institutions studying food safety and microbial quality, and certification bodies performing product testing. Laboratories use the procedures for routine checking of raw materials, finished food products and environmental swabs where coliform testing is required.

Related standards

Commonly referenced and related documents include successor and national variants for coliform testing such as GOST R 52816-2007 (document recommended as a later method for detection and enumeration of coliform bacteria), and other adjacent methods standards for Salmonella and general microbiological testing of foods.

Keywords

coliform, coli group, coliform bacteria, food microbiology, MPN, most probable number, plating, selective agar, enumeration, detection, food safety.

FAQ

Q: What is this standard?

A: GOST 30518-97 is a Russian national standard that defined methods for detecting and determining the number of coliform (coli group) bacteria in food products.

Q: What does it cover?

A: It covers sampling, presence/absence detection procedures and three quantitative approaches (MPN and two plating/enumeration methods on selective media), including incubation conditions and interpretation for routine food testing.

Q: Who typically uses it?

A: Food testing and quality-control laboratories, regulatory/public-health laboratories, research labs, and certification bodies involved in microbiological safety testing of foods.

Q: Is it current or superseded?

A: It is recorded as withdrawn / not in force in the Russian Federation and practical use has been superseded by later normative documents (for example GOST R 52816-2007 and other updated methods). Users should consult the current national or interstate standards and technical regulations for the applicable method.

Q: Is it part of a series?

A: It is part of the body of food microbiology test methods in the GOST/GOST R system; related methods include adjacent standards for detection of other pathogens (for example standards for Salmonella and for general microbiological counts).

Q: What are the key keywords?

A: coliform, coli group, food microbiology, MPN, enumeration, selective agar, detection, food safety.